Journal: bioRxiv
Article Title: Sorting nexin 5 mediates antigen presentation and immunity against Mycobacterium tuberculosis
doi: 10.64898/2026.01.30.702915
Figure Lengend Snippet: (A) Timeline of antigen presentation assay. (B) RAW 264.7, (C and D) BMDMs or (E and F) DCs, either primed or not with IFN-γ, were fed with OVA 323-339 , Mtb Ag85B, or heat-killed Mtb as depicted on the graphs. After (B) 4 or (C-F) 18h, the cells were incubated with antigen-specific MHC class II-restricted T cell hybridomas. After 24h, supernatants were assessed for IL-2 secretion by T cells. Bars are the mean ± S.D. from one of three experiments performed at least in triplicate. The circles shown correspond to each replica. *p < 0.05, **p < 0.01 and ***p < 0.001 (for indicated comparison; t-test ) .
Article Snippet: After overnight incubation, the cells were fed with vehicle or OVA 323-339 (the epitope of ovalbumin (OVA) presented by I-Ab, 5 mg/mL) (InvivoGen, 5825-43-01), Ag85B (10ng/mL) (Ray-Biotech, 228-11030 (discontinued) or BEI, NR5326), or heat-killed Mtb (MOI of 10) ( ).
Techniques: Immunopeptidomics, Incubation, Comparison